Control of Radiochemical Purity

Controle of radiochemical purity

Unavoidable control of radiochemical purity can really take just o couple of seconds thanks to the help of simple instruments. Using Lynax focusing screen enables you to observe the migration of the solution clearly.

Typical equipment for the controle of radiochemical purity

  1. Pad for chromatography PT298R0 2 pieces
  2. 2.Vial for chromathography or PT304R0 4 - 6 Pieces
  3. 3.Focusing screen PT234R01piece

See description of ceromended manipulation step by step:

Description of ceromended manipulation

1. A chromagraphical strip ITLC SG (the start, head and division having been marked previously - see the picture) is inserted into the Pad for chromatography PT298R0, the point “start” overhanging the edge of the pad at least 10 mm. 2ml of saline solution are to be added into the Vial for chromatography PT303R0 or PT304R0. A small volume of preparation (about the volume of its cone - 50μl) is withdrawn into a 2ml syringe. A small drop of preparation is applied on the start of the chromatogram (5 to 10μl).

Chromatographical strip

2. The chromatographical strip is now inserted into the Vial for chromatography PT303R0 or PT304R0 located on the pad of the Focusing screen PT234R0. The Pad for chromatography PT298R0 makes it easy avoiding touching of both working surface and chromatogram by hand. The migration of the solution can be clearly observed in a dissolved light of the Focusing screen PT234R0.

The chromatographical strip

3. The chromatographical strip is taken out of the vial when liquid phase achieves the head of the chromatogram. Simple tweezers are to be used. The strip is immediately divided into two parts in previously marked point (see the picture). Both strips are located into vials according to the chosen type of detection. The activities of both parts of chromatogram are measured now. Chromatographical classification of one preparation is to be executed three times and an everage value must be calculated to be compared with the total activity of both measured parts.